Colin G. Nichols

Address:
Department of Cell Biology and Physiology
Washington University School of Medicine
Box 8228
660 South Euclid Avenue
St. Louis, MO 63110, U.S.A.

Telephone: 314-362-6630
FAX No.: 314-362-7463

E-mail: cnichols at wustl dot edu

Education:

Scientific Education and Employment:

1982 1985 S.E.R.C.(CASE) graduate student, Department of Physiology,
University of Leeds (UK), immediate supervisor, Prof. B.R. Jewell.
1985 1988 Post doctoral Fellow, Dept. of Physiol., University of Maryland, laboratory of Prof. W.J. Lederer.
1988 1990 Research Associate, Department of Physiology, University of Maryland
1990-1991 Visiting Instructor, Department of Cell Biology, Baylor College of Medicine.
1990-1996 Visiting Research Assistant Professor, Department of Physiology, University of Maryland.
1991-1996 Assistant Professor, Department of Cell Biology and Physiology, Washington University School of Medicine.
1996-2000 Associate Professor, Department of Cell Biology and Physiology, Washington University School of Medicine.
2000- Professor, Department of Cell Biology and Physiology, Washington University School of Medicine..
2006- Carl Cori Professor, Washington University School of Medicine.

Honors and Awards:

CASE Studentship, Science and Engineering Research Council (U.K.) (1982 1985).
Research Fellowship, American Heart Association (1986 1988).
Established Investigator, American Heart Association (1993 1998).
Outstanding Faculty Mentor Award, Graduate School in Arts and Sciences, Washington University (2001).
Outstanding Faculty Mentor Award, Graduate School in Arts and Sciences, Washington University (2002).
Fellow of the American Heart Association (2001-).
Chair, Membrane Biophysics Sub-group, Biophysical Society (2003-2004).
Program Committee, Biophysical Society (2004-2005).
President, Society of General Physiologists (2006-2007).

Training/Teaching Responsibilities:

1982-1985 Tutor to First year Medical students
Ph.D. Thesis committee member: C.J. Solaro (Ph.D.,1995); T.J. Jegla (Ph.D.,1996); S. Tsunoda (Ph.D.,1996); J.C. Koster (Ph.D.,1996); R.E. Locke (Ph.D.,1996); D.M. Barry (Ph.D.,1997); M.Schreiber (Ph.D.,1998); M.W. Hill (Ph.D.,1999); S. Malin (Ph.D., 2001); J. Sehy (Ph.D., 2002); C.A. Remme (Ph.D., 2002, University of Amsterdam)
1988 Graduate student (D.L. Hamilton) supervisor
1989-1991 Post-Doctoral Fellow (C. Ripoll) supervisor. Research scientist, Univ. of Alicante
1991-1993 Clinical Fellow (J. Hardin) supervisor. Assistant Professor, Pediatrics, Univ. of Chicago
1992-1996 Post-Doctoral Fellow (A. Lopatin) supervisor. Assistant Professor, Dept. of Physiology, Univ. of Michigan
1992-2000 Post-Doctoral Fellow (E.N. Makhina) supervisor. Res. Asst. Prof., Dept. Pharmacology, Univ. of Pittsburgh
1992-1995 Coursemaster ("Ion channels: Targets for therapeutic intervention": Yr.1 Medical Student Elective Program).
1993-1994 Pre-Doctoral Howard Hughes Fellow (R. Baghdady) supervisor (MD, MA, May 1996). Clinician, California
1993-2004 Lecturer, Graduate neuroscience (Ion channels)
1993-1997, 2001 Lecturer, Medical Cell Biology (Electrophysiology)
1994-1998 Post-Doctoral Fellow (W. Pearson) supervisor. Res. Associate, Dept. of Cell Biology and Physiol. Wash .U.
1994-2001 Post-Doctoral Fellow (Q. Sha) supervisor. Res. Associate, Dept. of Mol. Biol. Pharmacol. Wash .U.
1995-1997 Post-Doctoral Fellow (S-L. Shyng) supervisor. Assistant Professor, Dept. of Physiology, Oregon Health Sci. U.
1996-2000 Post-Doctoral Fellow (J. Koster) supervisor. Res. Asst. Prof., Dept. of Cell Biology and Physiol. Wash .U.
1996-2002 Post-Doctoral Fellow (G. Loussouarn) supervisor. CNRS scientist, Univ. Nantes, France
1998-2001 Clinical Fellow (D. Enkvetchakul) supervisor. Instructor, Dept. of Medicine, Wash .U.
1998-2002 MD/PhD Graduate student (C. Cukras) supervisor (MD, PhD, May 2004). Resident in Medicine, Univ. Pennsylvania, Phila. PA.
1999-2004 PhD Graduate student (R. Phillips) supervisor (PhD, May 2005). Post-Doctoral Fellow, N.I.H. Bethesda, MD.
2000- Post-Doctoral Fellow (M-S. Remedi) supervisor
2001- 2005 Post-Doctoral Fellow (T. Flagg) supervisor. Res. Asst. Prof., Dept. of Cell Biology and Physiol. Wash .U.
2001- 2005 Post-Doctoral Fellow (J. Bhattacharya) supervisor
2002- MD/PhD Graduate student (R. Masia) supervisor
2003- 2005 Post-Doctoral Fellow (I. Jeliazkova) supervisor
2004- Post-Doctoral Fellow (H. Kurata) supervisor
2003- Lecturer, Neurology fellowship program
2003- Lecturer, Graduate Molecular Cell Biology program
2003- Lecturer, Graduate Signalling in the cardiovascular system

Memberships:

Biophysical Society (1989- ); American Heart Association Basic Science Council (1991- ); Juvenile Diabetes Foundation (1992- ); Society of General Physiologists (1993- ); Physiological Society of Great Britain (1996- ). American Diabetes Association (2002- )

Editorial and Administrative Responsibilities:

Editorial Board: Biophysical Journal (1998-2004)
Editorial Board: Circulation Research (1998-2003)
Editorial Board: Journal of General Physiology (1998-)
Guest Editor for Cardiovascular Research V28, No. 6 Special Issue on KATP channels (1994).
Reviewer for: Am. J. Physiol. (1989-), J. Physiol. (1990-), Pflugers Archiv. (1991-), Circulation Research (1991-), Circulation (1994-), Pediatric Research (1992- ), Journal of Cell Biology (1993- ), Journal of Membrane Biology (1993- ), J. Gen.Physiology (1994- ), TIPS (1994-), Neuron (1995- ), J. Biol. Chem.(1995- ), FASEB Journal (1995- ), Biophysical Journal (1995- ), J. Mol. Cell. Cardiol.(1995-), Science (1995- ), PNAS (1996-), EMBO J. (1997-), PNAS (1996-), EMBO J. (1997-), Biochemistry (Wash.) (1999- ), EMBO Reports (2000- ), Cell (2000- ), Nature Cell Biology (2000- ), Nature Medicine (2000- ), Molecular Pharmacology (2000- ), Nature (2002- ), Journal of Clin. Inv. (2002- )

Grants peer review committee member for Am. Heart Assoc. (Missouri Affiliate, 1993-1995; Great States Affiliate, 2000).
Permanent member NIH Physiology Study Section (1997-1998), NIH Molecular Cellular Developmental Neuroscience III Study Section (1998-2001).
Ad hoc reviewer for NIH Cardiovascular Study Section (1996-1997); NIH Molecular Cellular Developmental Neuroscience III Study Section (2001); NHLBI Program project review committee (2002); NIH Molecular aspects of diabetes and obesity Study Section (2005);
Ad hoc reviewer for Wellcome Foundation (UK), Wellcome Foundation (South Africa), and Medical Research Council of Great Britain (1994-).
Departmental representative and grant reviewer for McDonnell Center for Neuroscience at Washington University (1994-).
Departmental representative, Washington University Mouse Genetics Core Committee (2001-).
Faculty representative, Washington University Educational Policy Committee (2002-).
Abstract] reviewer, American Heart Association meeting, (2000).
Abstract] reviewer, American Diabetes Association meeting, (2002, 2003).
Academic Appointments/Promotions Committee, Washington University School of Medicine, Dept. of Anatomy and Neuroscience (2004).
Ad hoc reviewer for American Diabetes Association Research Grant Review Panel (2004).
Member American Diabetes Association Research Grant Review Panel (2004-2007).
Reviewer for NIDDK internal programs (2004).

PUBLICATIONS

Peer-reviewed articles:

1. Nichols, C.G. (1985). The influence of diastolic length on the contractility of isolated cat papillary muscle. Journal of Physiology, 361, 269 279. [Abstract]
2. Allen, D.G., Nichols, C.G. and Smith G.L. (1988) The effects of changes in muscle length during diastole on the calcium transient in ferret ventricular muscle. Journal of Physiology 406, 359-370. [Abstract]
3. Stern, M.D., Silverman, H.S., Houser, S.R., Josephson, R.A., Capogrossi, M.C. Nichols, C.G., Lederer, W.J. and Lakatta, E.G. (1988). Anoxic contractile failure in rat heart myocytes is caused by failure of intracellular calcium release due to alteration of the action potential. Proc. Natl. Acad. Sci., 85, 6954 6958. [Abstract]
4. Nichols, C.G., Hanck, D.A. and Jewell, B.R. (1988). The Anrep effect: An intrinsic myocardial mechanism. Canadian Journal of Physiology and Pharmacology, 66, 924 929. [Abstract]
5. Eisner, D.A., Nichols, C.G., O'Neill, S.C., Smith, G.L. and Valdeolmillos, M. (1989). The effects of interfering with metabolism on intracellular Calcium and pH in isolated rat ventricular cells. Journal of Physiology 411, 393-418. [Abstract]

6. Lederer, W.J., Nichols, C.G., and Smith, G.L. (1989). The mechanism of early contractile failure of isolated rat ventricular myocytes subjected to complete metabolic blockade. Journal of Physiology 413, 329-349. [Abstract]
7. Lederer, W.J. and Nichols, C.G. (1989). Nucleotide modulation of the activity of rat heart KATP channels in isolated membrane patches. Journal of Physiology 419, 193-211. [Abstract]
8. Nichols, C.G. and Lederer, W.J. (1990). The regulation of KATP channel activity in intact and permeabilized rat ventricular myocytes. Journal of Physiology 423, 91-110. [Abstract]
9. Nichols, C.G. and Lederer, W.J. (1990). The role of ATP in energy-deprivation contracture in unloaded rat ventricular myocytes. Canadian Journal of Physiology and Pharmacology 68, 183-194. [Abstract]
10. Nichols, C.G., Niggli, E. and Lederer, W.J. (1990). Modulation of ATP-sensitive potassium channel activity by flash-photolysis of 'caged-ATP' in rat heart cells. Pfluegers Archiv 415, 510-512. [Abstract]

11. Ripoll, C., Lederer, W.J. and Nichols, C.G. (1990). Modulation of ATP-sensitive K+ channel activity and contractile behavior in mammalian ventricle by the potassium channel openers cromakalim and RP49356. Journal of Pharmacology and Experimental Therapeutics 255, 429-435. [Abstract]
12. Nichols, C.G., Ripoll, C. and Lederer, W.J. (1991). KATP channel modulation of the guinea-pig ventricular action potential and contraction. Circulation Research 68, 280-287. [Abstract]
13. Cannell, M.B. and Nichols, C.G. (1991). The effects of pipette geometry on the time course of solution change in patch clamp experiments. Biophysical Journal 60, H1156-H1163. [Abstract]
14. Nichols, C.G., Lederer, W.J. and Cannell, M.B. (1991). The ATP-dependence of KATP channel kinetics in isolated membrane patches from rat ventricle. Biophysical Journal 60, 1164-1177. [Abstract]
15. Nichols, C.G. and Lederer, W.J. (1991). ATP-sensitive potassium channels in the cardiovascular system. American Journal of Physiology 261, H1675-H1686. [Abstract]

16. Cohen, N.M., Lederer, W.J. and Nichols, C.G. (1992). ATP-sensitive potassium channels underlie contractile failure in single human cardiac myocytes during complete metabolic inhibition. Journal of Cardiovascular Electrophysiology 3, 56-63.
17. Gudermann T., Nichols, C., Levy, F-O., Birnbaumer, M. and Birnbaumer, L. (1992). Ca2+ mobilization by the LH receptor expressed in Xenopus oocytes independent of cyclic AMP formation. Molecular Endocrinology 6, 272-278. [Abstract]
18. Aguilar-Bryan, L., Nichols, C.G., Rajan, A., Parker, C. and Bryan, J. (1992). Co-expression of sulfonylurea receptors and KATP channels in HIT T15 cells. Journal of Biological Chemistry 267, 14934-14940. [Abstract]
19. Rajan, A.S., Aguilar-Bryan, L., Nelson, D.A. Nichols, C.G., Wechsler, S.W., Lechago, J. and Bryan, J. (1992). Sulfonylurea receptors and ATP-sensitive K+ channels in clonal pancreatic alpha cells: Evidence for two high affinity sulfonylurea receptors. Journal of Biological Chemistry 268, 15221-15228. [Abstract]
20. Ripoll, C., Lederer, W.J. and Nichols, C.G. (1993). The mechanism of inhibition KATP channels by glibenclamide in rat ventricular myocytes. Journal of Cardiovascular Electrophysiology 4, 38-47. [Abstract]

21. Ho, K., Nichols, C.G., Lederer, W.J., Lytton, J., Vassilev, P.M., Kanazirska, M.V. and Hebert, S.C. (1993). Cloning and expression of an inwardly-rectifying, ATP-regulated potassium channel. Nature 362, 31-38. [Abstract]
22. Nichols, C.G. (1993). The 'inner core' of inward rectifier potassium channels. Trends in Pharmacological Sciences 14, 320-323.
23. Lopatin, A.N. and Nichols, C.G. (1993). 2,3-Butanedione Monoxime (BDM) inhibition of delayed rectifier (DRK1) Potassium channels expressed in Xenopus oocytes. Journal of Pharmacology and Experimental Therapeutics 265, 1011-1016. [Abstract]
24. Nichols, C.G. and Lopatin, A.N (1993). Trypsin and ?-chymotrypsin treatment abolishes glibenclamide sensitivity of KATP channels in rat ventricular myocytes. Pflugers Archiv. 422, 617-619. [Abstract]
25. Lopatin, A.N. and Nichols, C.G. (1994). Block of delayed rectifier (DRK1) K+ channels by internal 2,3-butanedione monoxime in Xenopus oocytes. Receptors and Channels 1, 279-286. [Abstract]

26. Nichols, C.G., Ho K. and Hebert, S. (1994). Mg2+ dependent inward rectification of ROMK1 potassium channels expressed in Xenopus oocytes. Journal of Physiology 476, 399-409. [Abstract]
27. Lopatin, A.N. and Nichols, C.G. (1994). Internal Na+ and Mg2+ blockade of DRK1 (Kv2.1) (Kv2.1) potassium channels. Journal of General Physiology 103, 203-216. [Abstract]
28. Makhina, E.N., Kelly, A.J., Lopatin, A.N., Mercer, R.W. and Nichols, C.G. (1994). Cloning and expression of a novel inward rectifier potassium channel from human brain. J. Biol. Chem. 269, 20468-20474. [Abstract]
29. Lopatin, A.N., Makhina, E.N. and Nichols, C.G. (1994). Potassium channel block by cytoplasmic polyamines as the mechanism of intrinsic rectification. Nature 372, 366-369. [Abstract]
30. Aguilar Bryan, L., Nichols, C.G., Wechsler, S.W., Clement, J.P. IV, Boyd, A.E. III, Gonzalez, G., Herrera Sosa, H., Nguy, K., Bryan, J. and Nelson, D.A. (1995). Cloning of the ? cell high affinity sulfonylurea receptor: a regulator of insulin secretion. Science 268, 423-426. [Abstract]

31. Ferrer, J., Nichols, C.G., Makhina, E.N., Salkoff, L., Bernstein, J., Gerhard, D., Wasson, J., Ramanadham, S. and Permutt, M.A. (1995). Pancreatic islets express a family of inwardly rectifying K channel subunits which interact to form G-protein activated channels. Journal of Biological Chemistry 270, 26086-26091. [Abstract]
32. Lopatin, A.N., Makhina, E.N. and Nichols, C.G. (1995). The mechanism of inward rectification of potassium channels. Journal of General Physiology 106, 923-955. [Abstract]
33. Sha, Q., Romano, C., Lopatin, A.N. and Nichols, C.G. (1996). Spermidine release from Xenopus oocytes: Electrodiffusion through a membrane channel. Journal of Biological Chemistry 271, 3392-3397. [Abstract]
34. Nichols, C.G., Shyng, S-L., Nestorowicz, A., Glaser, B., Clement, J.P. IV, Gonzales, G., Aguilar-Bryan, L., Permutt, A.M. and Bryan, J. (1996). Adenosine diphosphate as an intracellular regulator of insulin secretion. Science 272, 1785-1787. [Abstract]
35. Lopatin, A.N. and Nichols, C.G. (1996a). [K+]-dependence of open channel conductance in cloned inward rectifier potassium channels (IRK1, Kir2.1) Biophysical Journal 71, 682-694. [Abstract]

36. Lopatin, A.N. and Nichols, C.G. (1996b). [K+]-dependence of polyamine induced rectification in inward rectifier potassium channels (IRK1, Kir2.1). Journal of General Physiology 108, 105-113. [Abstract]
37. Henry, P., Pearson, W.L. and Nichols, C.G. (1996) Protein kinase C inhibition of cloned inward rectifier (HRK1/Kir2.3) K+ channels. J. Physiol. 495, 681-688. [Abstract only]
38. Nichols, C.G., Lopatin, A.N., Makhina, E.N., Pearson, W.L. and Sha, Q. (1996). Inward rectification and implications for cardiac excitability. Circulation Research 78, 1-7. [Abstract]
39. Shyng SL, Sha Q, Ferrigni T, Lopatin AN and Nichols CG. (1996). Depletion of intracellular polyamines relieves inward rectification of potassium channels. Proc.Natl.Acad.Sci. USA 93, 12014-12019. [Abstract]
40. Cohen, N.A., Sha, Q., Makhina, E.N., Lopatin, A.N., Linder, M.E., Snyder, S.H. and Nichols, C.G. (1996). Inhibition of an inward rectifier potassium channel by G-protein ?? subunits. Journal of Biological Chemistry 271, 32301-32305. [Abstract]

41. Shyng, S.L., Ferrigni, T. and Nichols, C.G. (1997). Control of rectification and gating of cloned KATP channels by the Kir6.2 subunit. Journal of General Physiology 110, 141-153. [Abstract]
42. Shyng, S.L., Ferrigni, T. and Nichols, C.G. (1997b). Regulation of KATP Channel Activity by Diazoxide and MgADP: Distinct Functions of the Two Nucleotide Binding Folds of the Sulfonylurea Receptor. Journal of General Physiology 110, 643-654. [Abstract]
43. Shyng, S.L. and Nichols, C.G. (1997). Octameric stoichiometry of the KATP channel complex. Journal of General Physiology 110, 655-664. [Abstract]
44. Nichols, C.G. and Lopatin, A.N. (1997). Inward rectifier potassium channels Annual Review of Physiology 59, 171-191. [Abstract]
45. Makhina, E.N. and Nichols, C.G. (1998). Independent trafficking of KATP channel subunits to the plasma membrane. Journal of Biological Chemistry. 273, 3369-3374. [Abstract]

46. Koster, J.C., Bentle, K.A., Nichols C.G., and Ho, K. (1998). Assembly of an inward rectifier K+ channel (ROMK1, Kir1.1a) involves multiple intersubunit association domains. Biophysical Journal 74, 1821-1829. [Abstract]
47. Lopatin, A.N., Makhina, E.N. and Nichols, C.G. (1998). A Novel Crystallization Method for Visualizing the Membrane Localization of Potassium Channels. Biophysical Journal 74, 2159-2170. [Abstract]
48. Lopatin, A.N., Makhina, E.N. and Nichols, C.G. (1998). Novel tools for localizing ion channels in living cells. Trends in Pharmacological Sciences 19, 395-398. [link]
49. Minor, N.T., Sha, Q., Nichols, C.G. and Mercer, R.W. (1998). The gamma subunit of the Na,K-ATPase induces cation channels. Proc. Natl. Acad. Sci. USA. 95, 6521-6525. [Abstract]
50. Shyng, S.-L., Ferrigni, T., Shepard, J.B., Nestorowicz, A., Glaser, B., Permutt, M.A. and Nichols, C.G. (1998). Functional analyses of novel mutations of the sulfonylurea receptor 1 associated with persistent hyperinsulinemic hypoglycemia of infancy. Diabetes. 47, 1145-1151. [Abstract]

51. Nestorowicz, A., Glaser, B., Wilson, B.A., Shyng, S.-L., Nichols, C.G., Stanley, C.A., Thornton, P.S. and Permutt, M.A. (1998). Genetic heterogeneity in familial hyperinsulinism. Human Molecular Genetics. 7, 1119-1128. [Abstract]
52. Pearson, W.L. and Nichols, C.G. (1998). Block of Kir 2.1 channels by alkylamine analogues of endogenous polyamines. J. Gen. Physiol. 112, 351-363. [Abstract]
53. Shyng, S.-L. and Nichols, C.G. (1998). Phosphatidyl inositol phosphates control of nucleotide-sensitivity of KATP channels Science. 282, 1138-1141. [Abstract]
54. Pearson, W.L., Dourado, M., Schreiber, M., Nichols, C.G. and Salkoff, L. (1999). Expression of a novel inward rectifier current from a Kir 4 family K+ channel gene cloned from mouse liver. Journal of Physiology 514, 639-653. [Abstract]
55. Koster, J.C., Sha, Q., Shyng, S-L. and Nichols, C.G. (1999). ATP Inhibition of KATP Channels: Control of nucleotide sensitivity by the cytoplasmic domains of the Kir6.2 subunit. Journal of Physiology 515, 19-30. [Abstract]

56. Koster, J.C., Q. Sha and C.G. Nichols (1999). Sulfonylurea and K+-channel opener sensitivity of KATP channels: Functional coupling of Kir6.2 and SUR1 subunits. J. Gen. Physiol. 114, 203-214. [Abstract]
57. Loussouarn, G., E.N. Makhina, T. Rose and C.G. Nichols (2000). Structure and dynamics of the pore of inward rectifier KATP channels. J. Biol. Chem. 275, 1137-1144. [Abstract]
58. Shyng, S-L., Barbieri, A., Gumusboga, A., Cukras, C., Pike, L., Davis, J.N., Stahl, P.D. and Nichols, C.G. (2000). Modulation of nucleotide sensitivity of KATP channels by PI-4-P 5-kinase. Proc. Natl.Acad. Sci. 97, 937-941. [Abstract]
59. Koster, J.C., B.A. Marshall, N. Ensor, J.A. Corbett and C.G. Nichols (2000). Targeted Overactivity of ?-cell KATP Channels Induces Profound Neonatal Diabetes. Cell 100, 645-654. [Abstract]
60. Enkvetchakul, D. G. Loussouarn, E. Makhina, S.L. Shyng and C.G. Nichols (2000). The kinetic and physical basis of KATP channel gating: Towards a unified molecular understanding. Biophys. J. 78, 2334-2348. [Abstract]

61. Gribble, F.M., Loussouarn, G., Tucker, S.J., Zhao, C., Nichols, C.G. and F.M. Ashcroft (2000). A novel method for measurement of sub-membrane ATP concentration. J. Biol. Chem. 275, 30046-30049. [Abstract]
62. Lopatin, A.N., L.M. Shantz, C.A. Mackintosh, C.G. Nichols and A.E. Pegg (2000). Modulation of potassium channels in the hearts of transgenic and mutant mice with altered polyamine biosynthesis. J. Mol. Cell. Cardiol. 32, 2007-2024. [Abstract]
63. Shyng, S.-L., C. Cukras, J. Harwood, and C.G. Nichols (2000). Structural determinants of phospholipid regulation of KATP channels. Journal of General Physiology 116, 599-608. [Abstract]
64. Enkvetchakul, D. G. Loussouarn, E. Makhina and C.G. Nichols (2001). ATP interaction with the open state of the KATP channel. Biophys. J. 80, 719-728. [Abstract]
65. Loussouarn, G., Phillips, L.R., Masia, R., Rose, T. and C.G. Nichols (2001). Flexibility of the Kir6.2 inward rectifier K+ channel pore. Proc. Natl. Acad. Sci. U.S.A. 98, 4227-4232. [Abstract]

66. Loussouarn, G., Pike, L., Ashcroft, F.M., Makhina, E.N. and C.G. Nichols (2001). Dynamic sensitivity of KATP channels to ATP. J. Biol. Chem 276, 29098-29103. [Abstract]
67. Lopatin, A.N. and Nichols, C.G. (2001) Inward Rectifiers in the Heart: An update on IK1. Journal of Molecular and Cellular Cardiology 33, 625-638. [Abstract]
68. Nerbonne, J.M., Nichols, C.G., Schwarz, T.L. and D. Escande (2001) Genetic Manipulation of Cardiac K+ Channel Function: What Have We Learned And Where Do We Go From Here? Circ. Res. 89, 944-956. [Abstract]
69. Sha, Q., Lansbery, K., Distefano, D., Mercer, R.W., and Nichols, C.G. (2001). Heterologous expression of the Na,K-ATPase gamma subunit in Xenopus oocytes induces an endogenous, voltage-gated large diameter pore. J. Physiol. 535, 407-417. [Abstract]
70. Koster, J.C., Knopp, A., Markova, K.P., Sha, Q., Enkvetchakul, D. Betsuyaku, T., Yamada, K. and C.G. Nichols (2001). Tolerance for reduced ATP-sensitivity of KATP channels in transgenic mice. Circ. Res.89, 1022-1029. [Abstract]

71. Luker, G.D., Flagg, T.P., Sha, Q., Luker, K.E., Pica, C.M., Nichols, C.G. and Piwnica-Worms, D.(2001). MDR1 P-glycoprotein Reduces Influx of Substrates Without Affecting Membrane Potential. J. Biol. Chem. 276, 49053-49060. [Abstract]
72. Cukras, C.A., Jeliazkova, I. and C.G. Nichols (2002). Structural and functional determinants of conserved inward rectifying K channel lipid interaction domains. J. Gen. Physiol. 119: 581-91. [Abstract]
73. Rajashree, R., Koster, J.C., Markova, K.P., Nichols, C.G. and Hofmann, P. (2002). Contractile function of hearts from transgenic mice with altered sarcolemmal KATP channels. Am. J. Physiol. 283, H584-H590. [Abstract]
74. Cukras, C.A., Jeliazkova, I. and C.G. Nichols (2002). The role of N-terminal positive charges in the activity of inward rectifier KATP channels. J. Gen. Physiol. 120, 437-446. [Abstract]
75. Koster, J.C., M.S. Remedi, T. Flagg, J. Johnson, K. Markova, B.A. Marshall & C.G. Nichols. (2002). Persistent hyperinsulinism induced by targeted suppression of ?-cell KATP channels. Proc. Natl. Acad. Sci. USA 99, 16992-16997. [Abstract]

76. Huopio, H., S-L. Shyng, T. Otonkoski and C.G. Nichols (2002). KATP channels and insulin secretion disorders. Am. J.Physiol. (Endo.) 283, E207–E216. [Abstract]
77. Loussouarn, G., T. Rose and C.G. Nichols (2002). Structural Basis of Inward Rectifying Potassium Channel Gating. Trends in Cardiovascular Medicine 12, 1195-1198. [Abstract]
78. Nichols, C.G. and J.C. Koster (2002). Diabetes and insulin secretion: Whither KATP? Am. J.Physiol. (Endo.) 283, E403-E412. [Abstract]
79. Phillips, L.R., D. Enkvetchakul & C.G. Nichols (2003). Gating-dependence of inner pore access in inward rectifier K+ channels. Neuron 37, 953–962. [Abstract]
80. Koster, J.C., M.S. Remedi, H. Qiu, C.G. Nichols & P.W. Hruz (2003). HIV Protease inhibitors acutely impair glucose-stimulated insulin release. Diabetes 52, 695-700. [Abstract]

81. Enkvetchakul, D. and C.G. Nichols (2003). Nucleotide-Gating of KATP channels: Function Fits Form. J. Gen. Physiol. 122, 471-480. [Abstract]
82. Phillips, L.R. and C.G. Nichols (2003). Ligand-induced closure of inward rectifier Kir6.2 channels traps spermine in the pore. Journal of General Physiology 122, 795-804. [Abstract]
83. Dibb, K., T. Rose, S.Y. Makary, T.W. Claydon, R. Leach, D. Enkvetchakul, C.G. Nichols and M.R. Boyett. (2003) Molecular basis of ion selectivity and block of inward rectifier Kir3.1/3.4 K+ channels. J. Biol. Chem. (278, 49537-49548. [Abstract]
84. Enkvetchakul, D., L. Ebihara and C.G. Nichols. (2003). Polyamine flux in Xenopus oocytes through hemi-gap junctions. J. Physiol. 553, 95-100. [Abstract]
85. Flagg, T.P., F. Charpentier, M.S. Remedi, M. McCluerie, A.N. Lopatin, J.C. Koster and C.G. Nichols (2003) Remodeling of excitation-contraction coupling in transgenic mice expressing ATP-insensitive sarcolemmal KATP channels.. Am. J.Physiol. (Heart) 286, H1361-9.

86. Manning-Fox, J.E., C.G. Nichols and P.E. Light (2004). Activation of ATP-sensitive potassium channels by acyl CoAs involves ATP- and PIP2-interacting residues. Mol.Pharm.. 18, 679-686. [Abstract]
87. Kurata, H.T., L.R. Phillips, T. Rose, G. Loussouarn, S. Herlitze, H. Fritzenschaft, D. Enkvetchakul, C.G. Nichols and T. Baukrowitz (2004) Molecular Basis of Inward-rectification: Polyamine interaction sites located by combined channel and ligand mutagenesis. J. Gen. Physiol. 124, 541-554. [Abstract]
88. Enkvetchakul, D., J. Bhattacharyya, K. Markova, D. Groesbeck, C. Cukras, I. Jeliazkova and C.G. Nichols (2004). Functional reconstitution of a bacterial Kir channel. J. Biol. Chem. 279, 47076-47080. [Abstract]
89. Remedi, M.S., J.C. Koster, K. Markova, S. Seino, T. Miki, B. Patton, M. McDaniel and C.G. Nichols (2004). Diet-induced glucose intolerance in mice with decreased beta-cell ATP-sensitive K+ channels. Diabetes 53, 3159-3167. [Abstract]
90. Flagg, T.P. and C.G. Nichols (2005) Sarcolemmal KATP Channels: What do we really know? Journal of Molecular and Cellular Cardiology 39, 61-70. [Abstract]

91. Loussouarn, G., L. Marton and C.G. Nichols (2005). Molecular Basis of Inward-rectification: Structural features of the blocker defined by extended polyamine analogs Molecular Pharmacology 68, 298-304. [Abstract]
92. Masia, R., D. Enkvetchakul and C.G. Nichols (2005) Preferential activation of Kir6.2 channels by SUR1 and SUR2A. J. Mol. Cell. Cardiol. 39, 491-501. [Abstract]
93. Koster, J.C., M.S. Remedi, C. Dao and C.G. Nichols (2005) ATP and sulfonylurea sensitivity of mutant ATP-sensitive K+ channels in neonatal diabetes: Implications for pharmacogenomic therapy. Diabetes 54, 2645–2654. [Abstract]
94. Makary, S.Y., T.W. Claydon, D. Enkvetchakul, C.G. Nichols and M.R. Boyett (2005). A difference in inward rectification and polyamine block and permeation between the Kir2.1 and Kir3.1/Kir3.4 K + channels J. Physiol. (Epub, 8/05). [Abstract]
95. Flagg, T.P., A. M. Remedi, R. Masia, J. Gomes., M. McLerie, A.N. Lopatin and C.G. Nichols (2005) Transgenic overexpression of SUR1 in the Heart suppresses sarcolemmal KATP. J. Mol. Cell. Cardiol. (Epub 8/05). [Abstract]

96. Prasad, S.M., A.S. Al-Dadah, G.D. Byrd, T.P. Flagg, J. Gomes, R.J. Damiano, C.G. Nichols and J.S. Lawton. (2005) The Role of the Sarcolemmal Adenosine Triphosphate – Sensitive Potassium Channel in Hyperkalemic Cardioplegia - Induced Myocyte Swelling and Reduced Contractility Annals of Thoracic Surgery (In press 6/05).
97. Remedi, M.S., B. Patton, M. McDaniel and C.G. Nichols (2005). Abrogation of glucokinase-deficiency diabetes (MODY2) in absence of KATP channels Diabetes (In press 7/05).
98. Koster, J.C. and C.G. Nichols (2005). Diabetes and insulin secretion: the KATP connection. Diabetes (In press 7/05).

Editorials, book reviews, other articles

99. Nichols, C.G. and Lederer, W.J. (1991). The mechanism of KATP channel regulation by ATP. Journal of General Physiology 97, 1095-1098. [link]
100. Baghdady, R. and Nichols, C.G. (1994). Controversy on drugs affecting ATP-sensitive potassium channels in ischemic heart disease. Cardiovascular Research 28, 135-136.
101. Nichols, C.G. (1994). Molecular biology of the KATP channel: Where's the clone? Cardiovascular Research 28, 727.
102. Nichols, C.G. (1995). Towards a structural model of the KATP channel. Heart and Vessels Suppl. 9, 5-7.
103. Shyng, S.L. and Nichols, C.G. (1999). Nucleotides and insulin secretion. Nutrition 15, 717-719. [link]
104. Nichols, C.G. and C.A. Cukras, C.A. (2001). KATP Channel regulators: Balanced diets include carbohydrates, proteins, and fats. Circulation Research 88, 849 - 851. [link]
105. Flagg, T.P. and Nichols, C.G. (2001). Sarcolemmal KATP Channels in the Heart: Molecular Mechanisms Brought to Light, but Physiological Consequences Still in the Dark. Journal of Cardiovascular Electrophysiology 12, 1195-1198. [Abstract]
106. Gutman, G.A., Chandy, K.G., Adelman, J.P., Aiyar, J., Bayliss, D.A., Clapham, D.E., Covarriubias, M., Desir, G.V., Furuichi, K., Ganetzky, B., Garcia, M.L., Grissmer, S., Jan, L.Y., Karschin, A., Kim, D., Kuperschmidt, S., Kurachi, Y., Lazdunski, M., Lesage, F., Lester, H.A., McKinnon, D., Nichols, C.G., O'Kelly, I., Robbins, J., Robertson, G.A., Rudy, B., Sanguinetti, M., Seino, S., Stuehmer, W., Tamkun, M.M., Vandenberg, C.A., Wei, A., Wulff, H., Wymore, R.S. (2004). International Union of Pharmacology. XLI. Compendium of voltage-gated ion channels: potassium channels. Pharmacol Rev. 55, 583-6. [Abstract]

Books

107. Lopatin, A.N. and C.G. Nichols, Eds. (2001). “Ion Channel Localization: Methods and Protocols”. Humana Press Inc. Totowa, New Jersey.

Book chapters

108. Allen, D.G., Smith, G.L. and Nichols, C.G. (1987). Intracellular calcium concentration following length changes in mammalian cardiac muscle. In `Starling's Law of the Heart revisited'. Ed. Ter Keurs, H.E.D.J., and Tyberg, J.V. Martinus Nijhoff, Holland.
109. Lederer, W.J., Berlin, J.R., Cohen, N.M., Nichols, C.G., Smith, G.L. and Cannell, M.B. (1988). Calcium current and excitation contraction coupling in heart. In `Lethal arrhythmias during anoxia and ischaemia'. Eds. M. Rosen and Y. Palti. Kluwer Academic., Boston.
110. Nichols, C.G., Lederer, W.J. and Ripoll, C. (1990). Competitive regulation of cardiac KATP channel activity. In `Ionic Currents and Ischemia'. Eds. J. Vereecke, P.P. Van Bogaert and F. Verdonck. Leuwen University Press, Belgium.
111. Nichols, C.G., Ripoll, C. and Lederer, W.J. (1990). How do KATP channels affect the cardiac action potential? In `Ionic Currents and Ischemia'. Eds. J. Vereecke, P.P. Van Bogaert and F. Verdonck. Leuwen University Press, Belgium.
112. Lederer, W.J. and Nichols, C.G. (1994). Regulation and function of adenosine-triphosphate-sensitive potassium channels in the cardiovascular system. In: 'Cardiac electrophysiology - From Cell to Bedside'. Eds. Zipes, D.P. and Jalife, J. W.B. Saunders Co. Phila., PA, USA.
113. Nichols, C.G., Cannell, M.B. and Lopatin, A.N. (1994) Cell-free ion channel recording. In "Patch clamp techniques and Protocols". Eds. A.A. Boulton, G.B. Baker and W.Walz. Humana Press Inc. Totowa, NJ, USA.
114. Nichols, C.G. (1995). Properties of cardiac ATP-sensitive potassium channels. In "Molecular physiology and pharmacology of cardiac ion channels and transporters". Ed. M. Morad. Kluwer Academic Publishers. Dordrecht, Holland.
115. Nichols, C.G. and Lopatin, A.N. (1998). Inward rectifiying potassium channels: ‘Mechanisms of rectification. In “Potassium ion channels: Molecular Structure, Function, and Diseases’. Eds. Y. Kurachi, L. Jan and Lazdunski, M. Academic Press. San Diego, CA, USA.
116. Nichols, C.G. (2000). Classical Inward-rectifier K channels: Mechanisms of inward rectification. In “Handbook of Experimental Pharmacology. Pharmacology of Ionic Channel Function”. Eds. M. Endo, M. Mishima and Y. Kurachi. Springer-Verlag. Heidelberg, Germany.
117. Koster, J.C. and C.G. Nichols (2001). Ch. 13. Tagging ion channels with the green fluorescent protein (GFP) as a method for studying ion channel function in transgenic mouse models. In: “Ion Channel Localization: Methods and Protocols.” Eds. Lopatin, A.N. and C.G. Nichols. Humana Press Inc. Totowa, New Jersey.
118. Makhina, E.N. and C.G. Nichols (2001). Ch. 14. Mutant GFP-based FRET analysis of K+ channel organization. In: “Ion Channel Localization: Methods and Protocols.” Eds. Lopatin, A.N. and C.G. Nichols. Humana Press Inc. Totowa, New Jersey.
119. Lopatin, A.N., Makhina, E.N. and C.G. Nichols (2001). Ch. 17. Crystallization technique for localizing ion channels in living cells. In: “Ion Channel Localization: Methods and Protocols.” Eds. Lopatin, A.N. and C.G. Nichols. Humana Press Inc. Totowa, New Jersey.
120. Shyng, S-L., A.N. Lopatin and C.G. Nichols (2002). Ch. 5. Molecular biology of inward rectifier and ATP-sensitive potassium channels. In: “Potassium channels in cardiovascular biology”. Eds. S. Archer and N. Rusch. Plenum Press, New York.
121. Lopatin, A.N. and C.G. Nichols. (2003). Inward rectifier potassium channels. In “Encyclopedic Reference of Molecular Pharmacology”, Eds. S. Offermanns and W. Rosenthal. Springer Verlag, Berlin, Germany.
122. Nichols, C.G., Koster, J.C., B.A. Marshall, and S.L. Shyng (2003). The role of KATP channels in regulation of insulin secretion. In “Molecular Insights into Ion Channel Biology in Health and Disease”. Ed. R. Maue. Elsevier Science, Amsterdam.
123. Kurata, H. and C.G. Nichols (2005). Polyamine block of inward-rectifier K+ channels: Towards a Structural Understanding. In “Polyamine Cell Signalling: Physiology, Pharmacology and Cancer Research”. Eds. J-Y Wang and R.A. Casero. Humana Press, Totowa, NJ.

Recent Invited Lectures (2000-2005):

• Structure-function relationships in cloned KATP channels - Dept. of Physiology, University of Washington, Seattle, WA, April 2001; Dept. of Physiology, Leeds University, U.K., July, 2001; American Diabetes Association 63rd Scientific Sessions, New Orleans, LA, June 2003.
• Polyamines and Potassium channels - Invited Speaker, Gordon Conference on Polyamines, Oxford, UK, September 2000; Invited Speaker, Gordon Conference on Polyamines, New London, Connecticut, June 2001.
• Diabetes caused by targeted transgene expression - Invited speaker, 20th European Winter Brain Conference, Lausanne, Switzerland, March 2000; Invited Speaker, Ion Channels Gordon Conference, Tilton, New Hampshire, July 2000; Invited speaker, Channelopathies, 2001 conference, Sheffield, UK, July 2001.
• Inward rectifier K channels: From molecular mechanisms to disease – Dept. of Physiology, Cornell University Medical School, NY, NY, December 2000; Dept. of Physiology, University of British Columbia, Vancouver, Canada, May 2001; Dept. of Physiology, University of Michigan, Ann Arbor, Michigan, October 2001; Ion Channels symposium, University of Colima, Colima, Mexico, November 2001; Dept. of Physiology, Yale University, New Haven, CT, Jan 2002; Dept. of Biological Sciences, Columbia University, New York, NY, Apr 2002; Invited speaker, World Congress of Cardiology, Nice, France, June, 2002; Physiological Society, Leeds, UK, September 2002; CMBN Retreat, University del Central Caribe, Puerto Rico, March 2003.
• Transgenic models of altered KATP channel function – Plenary lecture, Canadian Federation of Sciences, 43rd Annual meeting, Ottawa, June 2003. Invited speaker, Mammalian Myocardium meeting, Bristol, UK, July 2005.
• K channels: From structure to disease – Invited speaker, World Congress of Cardiology, Nice, France, June, 2004. Vollum Institute, Portland Oregon, September, 2004; Invited speaker Gerhard Giebisch retirement symposium, Yale University, New Haven CT, November 2004; Cornell College of Medicine, NY, March, 2005; UT San Antonio, April 2005; Dartmouth College, NH, May 2005; Univ. of Cardiff, Wales, UK, May 2005; Invited speaker, FASEB Conference on ‘Ion Channel Regulation’, Snowmass, CO, June 2005; Manchester University, UK, August 2005.
• KATP channels: The Diabetes connection – Dept. of Physiology, Vanderbilt University, Nashville, TN, January 2005.
• Phospholipids and K channels – Chair and Invited speaker, Combined Physiological Society/FEPS meeting, Bristol, UK, July 2005.